The X-Ray Crystallographic Core Facility is a recharge center shared by the Department of Chemistry and Department of Biochemistry. The facility specializes in single crystal X-ray diffraction and structure determination of small and macromolecules.
Access:
Users can access the facility by e-mailing Ryan VanderLinden, Director (r.vanderlinden@utah.edu). Samples can be dropped off at the facility or an arrangement for pickup can be made through e-mail. Users who have been trained to independently use the facility instruments may sign up for instrument time at http://resource.cores.utah.edu.
Services Offered:
Small Molecule Single Crystal X-ray diffraction
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- Unit Cell / Low Resolution Data w/ Unrefined Model – This service includes a low resolution data set used for accurate unit cell determination and if data is sufficient will result in low resolution structure.
- High Resolution Data w/ Unrefined Model. – This service will result in a high resolution data set and the necessary files (.ins and .hkl) for the user to complete model building and refinement.
- High Resolution Data w/ Refined Model and Structure Report. – This service will result in a high resolution data set with a completely refined model and a detailed report of the structure and experimental parameters. If desired the facility will deposit the structure in the CCDC CSD.
- Hourly Diffractometer Use– This service is offered for users who require crystal screening that does not result in the above services.
Macromolecule Single Crystal Diffraction.
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- Hourly Diffractometer Use– This service is offered for users who require crystal screening or data collection. For specimens that require longer exposures and/or extended data collection, a reduced rate for a 24-hour reservation is available.
Crystallization: Macromolecules and Water-Soluble Small Molecules.
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- ARI gryphon dropsetter hourly use
- Sparse matrix screens for high-throughput crystallization.
- Storage for Crystallization Plates (4C, 13C, 21C)
- Stereo microscopes (4C, 13C, 21C)
- Crystal optimization
- Crystal harvesting
- LN2 Storage for harvested Crystals
Crystallization: Solvent-Soluble Small Molecules.
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- Advice on crystallization of diffraction quality crystals (The facility is not currently equipped to support solvent-based crystallization.)
Rigaku XtaLAB Synergy DW
Single-crystal X-ray diffractometer featuring a microfocus rotating anode dual X-ray source(Mo Kα and Cu Kα), multilayer optics, HyPix hybrid photon-counting area detector, and an Oxford Cryostream 800 (80 K – 400 K).
ARI Gryphon automated dropsetter
The Gryphon automated drop setter (Art Robbins Instruments) is capable of high-precision nanoliter dispensing of protein and precipitant solutions for hanging- or sitting-drop vapor diffusion experiments. The drop setter allows for high throughput crystallization with sparse matrix crystallization screens for macromolecules and water soluble small molecules.
21 C / 13 C / 4 C Crystallization rooms
The X-ray facility has three isotemp rooms dedicated for crystallization growth. Each room has a stereomicroscope.
SSRL Synchrotron Access
For the most demanding crystallography projects, the X-ray facility can assist users in getting crystals to the SSRL for synchrotron X-ray diffraction data.
Facility Personnel:
Location
Eccles-Eccles Jones Medical Research Building (EEJMRB), RM 3700D
Directions to Facility:
The EEJMRB is on upper campus with the X-ray facility on the Southwest end of the 3rd floor.